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cold facs buffer for 10 min at 4 °c  (Miltenyi Biotec)


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    Miltenyi Biotec cold facs buffer for 10 min at 4 °c
    Cold Facs Buffer For 10 Min At 4 °C, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cold+facs+buffer+for+10+min+at+4+%C2%B0c/facs+buf10+min4/pmc11089003-288-25-19
    Average 90 stars, based on 1 article reviews
    cold facs buffer for 10 min at 4 °c - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Xenografted human microglia display diverse transcriptomic states in response to Alzheimer’s disease-related amyloid-β pathology
    Article Snippet: Following dissociation, myelin was removed by resuspending pelleted cells in 30% isotonic Percoll (GE Healthcare, Cat. no. 17-5445-02) and centrifuging at 300 g for 15 min at 4 °C. .. Accumulating layers of myelin and cellular debris were discarded and Fc receptors were blocked in FcR blocking solution (1:10, Miltenyi, Cat. no. 130-092-575) in cold FACS buffer for 10 min at 4 °C. .. Next, cells were washed in 5 ml of FACS buffer and pelleted cells were incubated with the following antibodies: PE-Pan-CD11b (1:50, Miltenyi, Cat. no. 130-113-806), BV421-mCD45 (1:500, BD Biosciences, Cat. no. 563890), APC-hCD45 (1:50, BD Biosciences, Cat. no. 555485), Total-Seq A cell hashing antibodies (1:500, BioLegend) and viability dye (1:2,000, eFluor 780, Thermo Fisher Scientific, Cat. no. 65-0865-14), in cold FACS buffer during 30 min at 4 °C.

    FACS:

    Article Title: Xenografted human microglia display diverse transcriptomic states in response to Alzheimer’s disease-related amyloid-β pathology
    Article Snippet: Following dissociation, myelin was removed by resuspending pelleted cells in 30% isotonic Percoll (GE Healthcare, Cat. no. 17-5445-02) and centrifuging at 300 g for 15 min at 4 °C. .. Accumulating layers of myelin and cellular debris were discarded and Fc receptors were blocked in FcR blocking solution (1:10, Miltenyi, Cat. no. 130-092-575) in cold FACS buffer for 10 min at 4 °C. .. Next, cells were washed in 5 ml of FACS buffer and pelleted cells were incubated with the following antibodies: PE-Pan-CD11b (1:50, Miltenyi, Cat. no. 130-113-806), BV421-mCD45 (1:500, BD Biosciences, Cat. no. 563890), APC-hCD45 (1:50, BD Biosciences, Cat. no. 555485), Total-Seq A cell hashing antibodies (1:500, BioLegend) and viability dye (1:2,000, eFluor 780, Thermo Fisher Scientific, Cat. no. 65-0865-14), in cold FACS buffer during 30 min at 4 °C.



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    cold facs buffer for 10 min at 4 °c  (Miltenyi Biotec)
    90
    Miltenyi Biotec cold facs buffer for 10 min at 4 °c
    Cold Facs Buffer For 10 Min At 4 °C, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cold+facs+buffer+for+10+min+at+4+%C2%B0c/facs+buf10+min4/pmc11089003-288-25-19
    Average 90 stars, based on 1 article reviews
    cold facs buffer for 10 min at 4 °c - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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